Firstly, with immunofluorescence assay, we found that the colocalization of FLOT1 with LAMP2 (lysosomal marker) (Figure 5E, G) and BHMT with LC3 (autophagosome marker) (Figure 5F, H) did occur in the irradiated GBM cells. The gene discussed is MAP1LC3A; the disease is glioblastoma.