Furthermore, Western blot assay (Figure 5I-J), mRFP-GFP-LC3 assay (Figure 5K-L) and immunofluorescence assay showed that FLOT1 and BHMT could also enhance the autophagy level in the irradiated GBM cells by increasing the fusion process of autophagosomes and lysosomes (Figure S4A). This evidence concerns the gene MAP1LC3A and glioblastoma.