Finally, the qRT-PCR results showed that RMST knockdown significantly downregulated the mRNA expression of the fibrotic genes, including Col1a1, Col3a1, fibronectin 1, and α-SMA, in TGF-β1-treated CFs (Figure 2M), which was in line with the change in Col1a1 and fibronectin 1 protein expression (Figure S3E-F). This evidence concerns the gene ACTA1 and myalgic encephalomeyelitis/chronic fatigue syndrome.