Obese ZDF rats developed severe NAFLD, the liver weight, liver index, and liver function including ALT and AST in the DM group were significantly higher than those in the NC group (P < 0.01), while these were significantly decreased in the EA group compared to the DM group (P <0.05, 0.01, Figures 2B–E). Here, GPT is linked to metabolic dysfunction-associated steatotic liver disease.