Cisplatin (DDP) is a promising anticancer drug, and cinobufagin combined with low‐dose DDP significantly inhibits OS cells activity and suppresses tumor growth and metastasis, thus increasing survival duration in an OS xenograft model of nude mice, while downregulating Notch 1, Hes 1, Hes 5, and Hey‐L mRNA expression.285. The gene discussed is NOTCH1; the disease is neoplasm.