To validate the cleavage of MAGED2 by Mpro in the virus infection condition, we infected human lung carcinoma A549 cells expressing human ACE2 (A549-hACE2) with SARS-CoV-2 and the cleaved products of MAGED2 could be observed at a multiplicity of infection (MOI) of 1 (Fig. 1F). Here, ACE2 is linked to viral infectious disease.