CCSCs are regulated via aberrant activation of the Wnt/β‐catenin, Notch and TGF‐β signaling pathways.[20, 21] The Wnt/β‐catenin signaling pathway exerts a key role in regulating the physiological functions and homeostasis of normal intestinal stem cells[22, 23, 24]; however, aberrant activation of the Wnt/β‐catenin signaling pathway drives the initiation of CRC, and promotes the maintenance and development of CRC via CCSCs,[25, 26] indicating that targeting the Wnt/β‐catenin axis may be an alternative method for treating CCSCs. The gene discussed is TGFB1; the disease is colorectal carcinoma.