In conclusion, in the present study we have characterized the role of GART in promoting CRC cell tumorigenicity and invasion via enhancing cancer stemness; furthermore, we have revealed the underlying mechanism through which GART activates Wnt/β‐catenin signaling, by methylating RUVBL1 to inhibit its ubiquitination degradation. This evidence concerns the gene RUVBL1 and colorectal carcinoma.