VCP and cyst: While we did observe protB-GFP expression in some VCP-RNAi canoe-stage spermatids (Figure 5c), we noted that protB-GFP was not uniformly expressed in all spermatid nuclei; strikingly, protB-GFP signal was present in some nuclei, but absent from adjacent nuclei within the same cyst (Figure 5c, arrows).