LASSO regression analysis was used to filter TFs further in order to identify a biomarker that could predict the prognosis of patients with melanoma (Figures 14C, D), based on which seven TFs (TBX21, MYB, GFI1, NFE2L3, TFAP2C, HEY2, NR2F2) were selected. The gene discussed is MYB; the disease is melanoma.