PIGG and infection: To test whether the c.1571C[7] and c.1571C[6] mga variants lost and regained function, emm mRNA levels were measured by real-time RT PCR for MGAS2221 (wt control), GAS1806 (c.1571C[7] mga variant of MGAS2221), and isolates M497, M405, and M406 from mouse infection with GAS1806.