The observed hypermethylation in the Pth-Cre:Men1 floxed/floxed was consistent with that reported in 12 patients with MEN1-associated hyperparathyroidism, when compared to 13 sporadic parathyroid adenomas, 4 parathyroid carcinomas, and 9 normal parathyroids, using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP), which specifically measures global 5mC marks (Kinney et al. 2011, Yuan et al. 2016). Here, MEN1 is linked to parathyroid gland carcinoma.