CD8A and neoplasm: T-cells from the r4T1-MBTA vaccine group showed significant cytotoxicity against 4T1 cells (over 60% 4T1 tumor cell death compared to the control), with partial cytotoxicity observed in splenocytes from the CD8-depleted r4T1-MBTA vaccinated group (around 42% 4T1 tumor cell death compared to the control) (Fig. 7e&f and supplementary Fig. 5b).