Next, we uncovered the spatial localization of the Invasive margin, through inference of copy number variations (CNVs), where the cellular tumor (CT) region was characterized by a gain of chromosome 7 (Additional file 1: Fig. S10F) and adjacent non-tumor (NT) region was delineated by high expression of RBFOX3 (NeuN) (Additional file 1: Fig. S10G). Here, RBFOX3 is linked to neoplasm.