Microglia (CD45-positive) and endothelial cells (CD31-positive) were depleted by FACS sorting to enrich the CD133-positive cells (N = 10,093 cells), which were subjected to scRNA-seq prior to characterization of glial progenitor cells (GP), oligo-lineage cells (OLC), and a mixed population including truncated radial glial cells and cancer mesenchymal cells. Here, PROM1 is linked to cancer.