Genes marked on the volcano plot were either implicated earlier in chordoma biology (e.g. keratins—KRT8, KRT18, KRT19, TBXT, LMX1A, and EGFR) or identified by outstanding p-value (e.g. IL11, CD24), high absolute fold-change (e.g. GABRA1) or DMR result (e.g. MNX1). The gene discussed is KRT8; the disease is chordoma.