Biochemical and cell biology approaches, such as RNA pull-down, western blot, immunostaining, and wound healing assays were used for evaluation of the impact of TWIST1/miR-145/ lnc-ZNF30-3 interactions in prostate cancer cells altered in miRNA and lncRNA expression.<h4>Results</h4>We identified a few potential lncRNA sponges of miR-145-5p, including lnc-ZNF30-3. Here, TWIST1 is linked to prostate cancer.