Validation of the variants and co-segregation in the family resulted in three variants located on chromosome 3 and co-segregating with AD: CCR3 p.F249Hfs*23 (rs561062190), ZNF589 p.T355M (rs376706270) and TLR9 p.E317D (novel) (Fig. 1 and SI Table 3). This evidence concerns the gene TLR9 and Alzheimer disease.