Therefore, we plan to observe the physiological signals during the dark period in APP/PS1 mice, and further differentiate waking into different levels of activity (such as active waking and quiet waking) to delve deeper into the role of autonomic nervous system during wakefulness in Alzheimer's disease and compare these findings with the experimental results obtained during the light period in the future study. This evidence concerns the gene APP and early-onset autosomal dominant Alzheimer disease.