We further investigated this relationship between CD34 and LSC17 scores at the single-cell level by examining cells obtained de novo from human patients with AML as well as purified human cells from patient-derived xenografts that were confirmed to self-renew by serial transplantation (a total of 6 samples from 5 patients; Figures S1C–S1E and Figure S2A–S2C). Here, CD34 is linked to acute myeloid leukemia.