Conversely, the EMT markers experienced a reversal in asthma in the Pycr1-KO model compared with the WT model ex vivo and in vitro, accompanied by fewer inflammatory cells, diminished secretion of mucus, decreased airway hyperresponsiveness, and reduced collagen I. Hence, elevated proline may promote EMT-mediated airway remodeling through an increase in PYCR1 in a chronic asthma model. This evidence concerns the gene PYCR1 and chronic asthma.