Since long-chain fatty acids, including myristic acid, stimulate the excessive influx of fatty acids into hepatocytes and induce the increase of pro-inflammatory cytokines and NF-κB, contributing to hepatic inflammation, our findings identified a previously unknown link between microbial and metabolomic alterations in obesity, suggesting myristic acid as a possible target for treating pediatric obesity. Here, NFKB1 is linked to obesity due to melanocortin 4 receptor deficiency.