PDCD1 and neoplasm: Metascape analysis was performed on the DEGs of ACTA2+ myofibroblasts from the PD-1+SMI group, and it was found that the regulation of intrinsic apoptosis signaling pathways was enriched (Additional file 1: Fig. S14B), indicating that the ACTA2+ myofibroblasts in the PD-1+SMI group may be in a state of apoptosis, and SMI assisting PD-1 inhibitor may inhibit the effect of the ACTA2+ myofibroblasts on tumor progression.