Consistently, the tumor-infiltrating GZMAhigh NK subcluster expressing a high level of cytotoxic genes (GZMA, GZMB, GZMK, GZMM, GZMH, PRF1, and GNLY) and FASL and TRAIL genes was most enriched in the PD-1+SMI group. This evidence concerns the gene PRF1 and neoplasm.