To determine the clinical significance of EGFR/EZH2 dependent NFAT5 lysine methylation in glioma, we performed IHC analysis to examine the expression and correlation between EZH2 pS21 and Me3-NFAT5 K668 as well as EGFR pY1068 levels in 83 human glioma specimens. This evidence concerns the gene EGFR and central nervous system cancer.