Interestingly, a previous study demonstrated THY1 expression was downregulated in ectopic SIX1 overexpress fibroblast tumors in RNA-Seq analysis.28 Zhang et al19 revealed that CD90, a gene product of THY1 and a marker of HCC CSCs, and SIX1 suppression affected the CD90 positive cell count in the study. This evidence concerns the gene SIX1 and hepatocellular carcinoma.