The current preliminary AOP for microcephaly (AOP 441) is composed of the following KEs: “DNA damage” inducing “Activation of TP53” leading to “Apoptosis” and “Premature cell differentiation” leading to “Microcephaly.” These findings confirm the efficiency of the automated strategy to identify known KE relevant for AOP development (Figure 3; Supplementary Figure S2). The gene discussed is TP53; the disease is microcephaly.