Then, we performed comprehensive analysis of GJB2 expression in 33 different cancers and adjacent normal tissues using the Sangerbox database and found that GJB2 was overexpressed in GBM, UCEC, BRCA, CESC, LUAD, ESCA, STES, KIRP, KIPAN, COAD, COADREAD, PRAD, STAD, KIRC, LUSC, BLCA, THCA, OV, PAAD, UCS, and ALL but downregulated in LGG, LIHC, SKCM, TGCT, LAML, PCPG, ACC, KICH, and CHOL (Supplementary Figure 1). This evidence concerns the gene GJB2 and cancer.