In a lung tissue simulation system constructed from differentiated THP-1 macrophages and human non-small cell lung cancer A549 cells, treatment with CuONPs resulted in upregulation of the chemokine C-C motif chemokine ligand 22 (CCL22) and IL-1β genes in A549 cells and increased superoxide dismutase 2 (SOD2) expression. The gene discussed is SOD2; the disease is non-small cell lung carcinoma.