On the other hand, other tumor MCF/Neo, MCF7/HER2, HCC1954 and BT474 tumors grown in nude mice, and HCC1954 and BT474 grown in NSG mice showed significant difference in positivity from periphery to the center, mirroring that of Prussian blue stained pattern(s) of quartile positivity (Figure 6). The gene discussed is ERBB2; the disease is neoplasm.