In addition, they performed specific activity tests for L-glutamine, presenting 0.38% of L-asparaginase activity, and cytotoxicity tests on MOLT-4 leukemia cells, killing 85% of the cells under physiological conditions (pH 7.4 at 37°C), with optimal activity at pH 8.6 and at 40°C. Here, ASPG is linked to leukemia.