TP53 and neoplasm: The antitumor effect includes several steps to understand how the extract works on these tumor cell lines, including cytotoxicity, antiproliferative, changes in mitochondrial membrane potential (ΔΨm) and plasma membrane integrity, its effect on cell cycle distribution, induction of apoptosis, and determination of apoptotic biomarkers such as caspase 3 and the tumor suppressor protein p53.