BAG1 and cyst: In our research, in the brain homogenate of the mice infected with ME49Δgra5, no cyst was detected at 75 dpi (Figure 1D); the BAG1 protein band was detected weaker and weaker by WB at 15 and 30 dpi and became undetectable at 75 dpi (Figure 1F), but the B1 gene which is present in both tachyzoites and bradyzoites replicated significantly at 15 dpi, compared with 7 and 30 dpi, and then became undetectable at 75 dpi (Figure 1G).