In brief, CFSE-labelled CD4+ and CD8+ T cells sorted ex vivo from CRC samples were live-stained with stably fluorescently conjugated anti-TMEM123 antibody (Qdot-705) and afterwards incubated with HT-29 cells grown to pre-confluency and monitored over a 2-day recording of 3-hour time-lapse experiment (Figure 6A). Here, CD8A is linked to colorectal carcinoma.