Finally, the B16F10-OVA-elicited T cell activation in the lung-draining LNs was specific to CD8+ T cells, as there was no detectable difference in activated CD4+ T cell or CD4+ TEM frequencies within wild-type or E2-/- tumor-draining mediastinal LNs (Supplemental Figures 2H, I). The gene discussed is CD4; the disease is neoplasm.