Interestingly, Ly6Clo monocytes within the lung-draining medLNs were phenotypically distinct from circulating nonclassical monocytes in that they displayed decreased CD16.2, Treml4, and CX3CR1 expression (Figure 5D), suggesting that alterations in monocyte phenotype occur within these tumor-draining lymph nodes. This evidence concerns the gene TREML4 and neoplasm.