HDM induced DC activation through TLR4, activating Th2 response in an adjuvant-free murine model; the use of TLR4 antagonist (underacyletd form of R. spharoides LPS) reduced airway inflammation to the baseline level of non-sensitized mice, in terms of eosinophilia and lymphocytosis (Hammad et al., 2009). The gene discussed is TLR4; the disease is Increased total eosinophil count.