Some reports have revealed that HIF‐1α can significantly upregulate pluripotent gene expression, such as SOX2, NONAG, OCT4, and KLF4 to restrain cell differentiation and keep the stemness phenotype.[21] We evaluated the mRNA expression of stemness genes in all groups and we found the expression of ICAM‐1 (Figure 6A), SOX2 (Figure 6B), and CDCA8 (Figure 6C) could be significantly upregulated in PDA‐modified group. This evidence concerns the gene KLF4 and Patent ductus arteriosus.