In this study, we found that HMGB-1 expression and translocation were increased in the cardiomyocytes of the HFD-STZ diabetes untreated rats, which conforms to the previous observation of Wang et al.58 who proposed that HMGB-1 is related to diabetes-associated myocardial dysfunction and inhibition of HMGB-1 might have potential prospectives in the treatment of DCM. This evidence concerns the gene HMGB1 and diabetes mellitus.