Multifactorial analysis showed that the model with pathological grading, AFP, surgical treatment, T-stage, tumor size, and M-stage had the smallest AIC values, but since pathological grading significantly affects overall survival, we included AFP, surgical treatment, T-stage, M-stage, and tumor size as influencing survival of non-cirrhotic hepatocellular carcinoma (HCC-NCL) patients independent prognostic factors and entered into a columnar graphical model for analysis (Table 2). This evidence concerns the gene AFP and hepatocellular carcinoma.