We selected baseline date observed in the overall cohort or variables that may interfere with the results as co-variables for matching, and the covariates included age, gender, BMI, ASA score, tumor location, TNM staging, carcinoembryonic antigen (CEA) level, receiving of neoadjuvant chemoradiotherapy (nCRT), LCA retention and ICG angiography. The gene discussed is CEACAM5; the disease is neoplasm.