To further demonstrate the high cytotoxicity of the CD8+ T cells upon treatment of LPS-Nb36 and DC/tumor fusion vaccine against corresponding target cells, we measured the IFN-γ intracellular expression by flow cytometry and the mobilization of CD107a (representing the cytotoxic phenotype) across the membrane of these CD8+ T cells, The findings unveiled an upregulated IFN-γ and CD107a expression in DC/tumor fusion + LPS-Nb36 groups (Fig. 5B), which is consistent with the outcomes of the cytotoxicity assay. The gene discussed is CD8A; the disease is neoplasm.