As expected, in this work, the CD8+ T cells activated by DC/tumor fusion vaccine in a combination of LPS-Nb36 could rapidly transmit T cell activation signals after encountering tumor antigens, as demonstrated by upregulated CD25 and CD69 expression, and enhanced secretion of cytokines (TNF-α, IFN-γ, and IL-2). This evidence concerns the gene TNF and neoplasm.