Using three different cell lines as biological replicates, we further evaluated the kinases essential for human osteosarcoma cells, revealing 26 significant candidate hits (beta score < −0.5 and false discovery rate (FDR) < 1%) (Fig. 1F and Table S1), which were enriched in pathways associated with cell cycle and TP53-regulated transcription (Fig. 1G). Here, TP53 is linked to osteosarcoma.