To further elucidate the effect of YBX3 on the progression of ccRCC in vitro, YBX3 was overexpressed in the ACHN cell line, which expressed only a low level of YBX3, and the overexpression was confirmed via qRT-PCR and western blot analyses (Fig. 7A-B, P < 0.001). This evidence concerns the gene YBX3 and nonpapillary renal cell carcinoma.