Next generation sequencing (NGS) analysis showed low tumour mutational burden (TMB) and stable microsatellite instability (MSI) status and detected a BRAF p.V600E (c.1799T>A) mutation with a variant allele frequency (VAF) of 49% in a sample with histologically estimated tumour cell ratio of 85% (Figure 1B). This evidence concerns the gene BRAF and neoplasm.