CARD8 and HIV-1 infection: To further evaluate the role of the human-derived F59-F60 motif of CARD8 after HIV-1 infection, we used a doxycycline (dox)-inducible system to complement CARD8 KO THP-1 cells with either WT CARD8 or CARD8 cleavage mutants (Figure 5A) and probed for subsequent inflammasome activation.