The flow cytometry data indicated that coculture of Jurkat, an immortalized T lymphocyte cell line, with BT-549 tumor cells expressing anti-CD3 resulted in a notable increase of the T cell activation marker CD69, compared to its levels in BT-549-Vn coculture (Figure 4C and 4D), suggesting that T cells were activated via their T cell receptors triggered by anti-CD3 engineered on tumor cells. This evidence concerns the gene CD69 and neoplasm.