Briefly, given that T cells are activated via CD3, we generated human breast cancer BT-549 cell lines that stably express an empty vector (BT-549-Vn) or a construct encoding an anti-CD3 antibody fragment fused to the transmembrane domain of CD14 (BT-549-αCD3), and the expression of anti-CD3 was examined with a CD14 antibody (Figure 4A and 4B). Here, CD14 is linked to breast cancer.