To test the possibility that RNase1-mediated T cell activation can affect T cell-mediated killing function, as shown in Figure 5A and 5B, we performed a T cell-mediated tumor cell-killing assay by coculturing human PBMCs with BT-549-Vn or BT-549-αCD3 cells expressing nuclear-restricted red fluorescent protein (red nuclei indicated live cells) in the presence of caspase 3/7 substrate treated with or without RNase1 and then measured green fluorescent caspase-3/7 activity, representing apoptotic dead cells. This evidence concerns the gene CASP3 and neoplasm.