Another research investigated the optimal combination of different cytokines, and demonstrated that a mixture of mRNAs encoding IL-12 single chain, interferon-α (IFN-α), granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-15 increased systemic antigen-specific T-cell expansion and granzyme B T-cell infiltration, thereby promoting tumor regression 177. The gene discussed is CSF2; the disease is neoplasm.