However, since the relationship between DAP3, GSDME, PPP2R5B, and PLK1 expression and HCC has not been previously reported, we performed the qRT-PCR analysis in LO2, HepG2, and HL-7721 cells and showed that GSDME, PPP2R5B, and PLK1 expression was upregulated in HCC cell lines. The gene discussed is DAP3; the disease is hepatocellular carcinoma.