To confirm whether YWHAH can affect the proliferation of GC cells by positively regulating Fra-1 to activate the HMGA1/PI3K/AKT/mTOR signaling pathway, we first transfected NC+siNC, OE-YWHAH+siNC, and OE-YWHAH+siFra-1 plasmids into SGC7901 GC cells, and detected the mRNA levels of PI3K/AKT/mTOR signal pathway related molecules (PI3K, AKT, PDK1, MTOR, etc.)using qRT-PCR. This evidence concerns the gene FOSL1 and gastric cancer.