The results indicated that compared with the scrambled control, the miR-4256 mimic induced a significant increase in the relative luciferase activity of the pGL4-HDAC5-WT reporter, but the relative luciferase activity of the pGL4-HDAC5-MUT reporter was not significantly affected by miR-4256 mimic transfection in GC cells (SGC-7901 and HGC-27 cells). The gene discussed is HDAC5; the disease is gastric cancer.