HDAC5 and gastric cancer: CCK-8 and Transwell assays revealed that transient overexpression of HDAC5 exacerbated proliferation, migration, and invasion in GC cells compared with the HDAC5-negative control or the scrambled control; of note, the combination of transient transfection of HDAC5 and miR-4256 inhibitor eliminated the inhibition of malignant biological behavior caused by the miR-4256 inhibitor (Figs. 3D, 3E and Suppl.