To test this hypothesis, we conducted rescue experiments by treating LLNLR-299G3.1-overexpression ESCC cells with si-RNAs for cytokine-cytokine receptor interaction pathway gene (TNFRSF4) and neuroactive ligand-receptor interaction pathway gene (HRH3), respectively. This evidence concerns the gene TNFRSF4 and esophageal squamous cell carcinoma.