To further illustrate the mechanism of miR-33a-3p and IGF2 in osteoporosis, hBMSCs were transfected with mimic control, miR-33a-3p mimic, miR-33a-3p mimic + control-plasmid or miR-33a-3p mimic + IGF2-plasmid for 48 h and then stimulated with osteogenic induction medium for 14 days. This evidence concerns the gene IGF2 and osteoporosis.