EP300 and infection: To verify the role of P300 in HASMC ferroptosis, we first generated two different short-hairpin RNA (shRNA) plasmids to knockdown P300 (shP300-1and shP300-2) and considering the nature of the P300 transcriptional co-activator acting in the nucleus, we tested the total and nuclear protein expression level of P300 in HASMCs after infection with indicated lentiviruses.