To examine whether the increased AT1R expression in RVLM astrocytes of SHRSPs was associated with their development of hypertension, we manipulated AT1R signalling by introducing exogenous expression of AT1R-associated protein (AGTRAP), which interacts with AT1R and tempers the angiotensin-II-mediated signals by promoting AT1R internalization35. This evidence concerns the gene AGTR1 and hypertensive disorder.